Hotspot

petA-psbJ

LSC · 65425-66581 · 1157 bp

Back to Hippeastrum

Pi 0.00627

MarkerSeek score 49.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1157
variable_sites: 16
pi: 0.00627
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTGGC	CCGGATTGGGTTCATCTCTG	50.0	55.0	57.8	58.0	1269-1291 bp	1.0	80.4
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	TCCGGATTGGGTTCATCTCT	50.0	50.0	57.8	58.1	1270-1292 bp	1.0	79.7
petA-psbJ_p3	GGCCTTTTGTTCTTCTTGGC	GCTGGTATTCCTGTGATCGG	50.0	55.0	57.8	58.1	1321-1343 bp	1.0	79.6
petA-psbJ_p4	CGTCCAAGGCCTTTTGTTCT	CCGGATTGGGTTCATCTCTG	50.0	55.0	58.7	58.0	1276-1298 bp	1.0	76.1
petA-psbJ_p5	CGTCCAAGGCCTTTTGTTCT	TCCGGATTGGGTTCATCTCT	50.0	50.0	58.7	58.1	1277-1299 bp	1.0	75.5