Hotspot

rbcL-accD

LSC · 59080-59877 · 798 bp

Back to Hippeastrum

Pi 0.00418

MarkerSeek score 51.40

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 798
variable_sites: 12
pi: 0.00418
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CGCTGCTTGTGAAGTATGGA	CAACATCGAATTGAACCGCC	50.0	50.0	58.3	58.1	883-909 bp	1.0	81.0
rbcL-accD_p2	CGCTGCTTGTGAAGTATGGA	ACATCGAATTGAACCGCCAT	50.0	45.0	58.3	58.2	881-907 bp	1.0	79.8
rbcL-accD_p3	AAATGGAGCCCTGAACTAGC	CAACATCGAATTGAACCGCC	50.0	50.0	57.6	58.1	903-929 bp	1.0	79.7
rbcL-accD_p4	CGCTGCTTGTGAAGTATGGA	CCGGTATGTCCAAGAGCATT	50.0	50.0	58.3	57.7	957-983 bp	1.0	78.8
rbcL-accD_p5	AAATGGAGCCCTGAACTAGC	ACATCGAATTGAACCGCCAT	50.0	45.0	57.6	58.2	901-927 bp	1.0	78.5