Hotspot

rpoC2

LSC · 27094-31647 · 4554 bp

Back to Himalayacalamus

Pi 0.00154

MarkerSeek score 50.60

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4554
variable_sites: 7
pi: 0.00154
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	TTCGCTCCACAAGGATCAAG	TCCGCCTCTTCAGGAAAATG	50.0	50.0	57.9	57.9	1137 bp	1.0	78.5
rpoC2_p2	AAGCGAAGAAATGGGTTCGT	TCCGCCTCTTCAGGAAAATG	45.0	50.0	58.1	57.9	902 bp	1.0	78.5
rpoC2_p3	TGACGATCCACGATACAGAA	CCCATATTCGTCTTCTAGGGC	45.0	52.4	56.1	57.7	262 bp	1.0	55.2
rpoC2_p4	TTGACGATCCACGATACAGA	CCCATATTCGTCTTCTAGGGC	45.0	52.4	56.1	57.7	263 bp	1.0	55.2
rpoC2_p5	TGACGATCCACGATACAGAA	TGAGTCTTTCTCTCCAGTCCT	45.0	47.6	56.1	57.5	301 bp	1.0	53.4