Hotspot

ccsA

SSC · 109166-110143 · 978 bp

Back to Himalayacalamus

Pi 0.00307

MarkerSeek score 60.50

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 978
variable_sites: 3
pi: 0.00307
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GCGGCATTCTCGAAAAAGAA	TTTGAGAAGGCGTTCAAGGG	45.0	50.0	57.4	58.4	1140 bp	1.0	72.2
ccsA_p2	GCGGCATTCTCGAAAAAGAA	GGCGTTCAAGGGTTCTCAAA	45.0	50.0	57.4	58.7	1132 bp	1.0	69.8
ccsA_p3	GCGGCATTCTCGAAAAAGAA	GAAGGCGTTCAAGGGTTCTC	45.0	55.0	57.4	58.8	1135 bp	1.0	68.6
ccsA_p4	GCGGCATTCTCGAAAAAGAA	GAGAAGGCGTTCAAGGGTTC	45.0	55.0	57.4	58.8	1137 bp	1.0	68.6
ccsA_p5	GCGGCATTCTCGAAAAAGAAT	TTTGAGAAGGCGTTCAAGGG	42.9	50.0	57.9	58.4	1140 bp	1.0	68.0