Hotspot

psaJ-rpl33

LSC · 67178-67627 · 450 bp

Back to Himalayacalamus

Pi 0.00444

MarkerSeek score 58.20

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 450
variable_sites: 2
pi: 0.00444
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTTGGGGCTTTAGCAGGTTT	GAAATCCCCGTCGATTCCTC	45.0	55.0	57.9	58.1	617 bp	1.0	78.4
psaJ-rpl33_p2	TTTGGGGCTTTAGCAGGTTT	GGCACCTTTTCGAACACAAC	45.0	50.0	57.9	58.2	594 bp	1.0	78.2
psaJ-rpl33_p3	TTTGGGGCTTTAGCAGGTTT	CGATTCCTCATTGGCACCTT	45.0	50.0	57.9	58.2	606 bp	1.0	77.5
psaJ-rpl33_p4	TTTGGGGCTTTAGCAGGTTT	CTAACACCTTTCCCCTTGGC	45.0	55.0	57.9	58.5	548 bp	1.0	75.9
psaJ-rpl33_p5	TTTGGGGCTTTAGCAGGTTT	CCTCATTGGCACCTTTTCGA	45.0	50.0	57.9	58.5	601 bp	1.0	75.8