Hotspot

trnL-UAA-trnF-GAA

LSC · 49696-50041 · 346 bp

Back to Himalayacalamus

Pi 0.0116
MarkerSeek score 60.10
Primer available yes
Label trnL-UAA-trnF-GAA

Feature Metadata

label_name
trnL-UAA-trnF-GAA
feature_type
igs
region
LSC
length_bp
346
variable_sites
4
pi
0.0116
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAA-trnF-GAA_p1 GTTGTGAGGGTTCAAGTCCC TTTGAACTGGTGACACGAGG 55.0 50.0 58.4 58.1 435 bp 1.0 77.2
trnL-UAA-trnF-GAA_p2 GTTGTGAGGGTTCAAGTCCC CTGTGCCAGGAACCAGATTT 55.0 50.0 58.4 58.1 452 bp 1.0 76.9
trnL-UAA-trnF-GAA_p3 GTTGTGAGGGTTCAAGTCCC GTGCCAGGAACCAGATTTGA 55.0 50.0 58.4 58.1 450 bp 1.0 76.8
trnL-UAA-trnF-GAA_p4 GTTGTGAGGGTTCAAGTCCC TTTTTCTGTGCCAGGAACCA 55.0 45.0 58.4 57.8 457 bp 1.0 76.5
trnL-UAA-trnF-GAA_p5 AGTTGTGAGGGTTCAAGTCC TTTGAACTGGTGACACGAGG 50.0 50.0 57.4 58.1 436 bp 1.0 75.2