Hotspot

trnG-UCC-trnT-GGU

LSC · 14340-16228 · 1889 bp

Back to Himalayacalamus

Pi 0.00318

MarkerSeek score 52.80

Primer available yes

Label trnG-UCC-trnT-GGU

Feature Metadata

label_name: trnG-UCC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1889
variable_sites: 6
pi: 0.00318
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnT-GGU_p1	ACGAATCACACTTTTACCACT	CCATGGCATTACTCTACCACC	38.1	52.4	55.5	58.1	2020-2108 bp	1.0	42.8
trnG-UCC-trnT-GGU_p2	AGAACGAATCACACTTTTACCAC	CCATGGCATTACTCTACCACC	39.1	52.4	57.3	58.1	2023-2111 bp	1.0	41.7
trnG-UCC-trnT-GGU_p3	GAACGAATCACACTTTTACCACT	CCATGGCATTACTCTACCACC	39.1	52.4	57.3	58.1	2022-2110 bp	1.0	41.7
trnG-UCC-trnT-GGU_p4	ACGAATCACACTTTTACCACT	TGAACCGATGACTTATGCCT	38.1	45.0	55.5	56.6	1975-2063 bp	1.0	41.0
trnG-UCC-trnT-GGU_p5	AACGAATCACACTTTTACCACT	CCATGGCATTACTCTACCACC	36.4	52.4	56.2	58.1	2021-2109 bp	1.0	40.8