Hotspot

trnfM-CAU-trnG-UCC

LSC · 13510-13599 · 90 bp

Back to Himalayacalamus

Pi 0.0222

MarkerSeek score 65.30

Primer available yes

Label trnfM-CAU-trnG-UCC

Feature Metadata

label_name: trnfM-CAU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 90
variable_sites: 2
pi: 0.0222
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnfM-CAU-trnG-UCC_p1	CCTCAAGGTTATGAGCCTCG	AGCCTTCCAAGCTAACGATG	55.0	50.0	57.8	58.0	181 bp	1.0	78.8
trnfM-CAU-trnG-UCC_p2	CCTCAAGGTTATGAGCCTCG	CCTAGCCTTCCAAGCTAACG	55.0	55.0	57.8	58.1	184 bp	1.0	78.6
trnfM-CAU-trnG-UCC_p3	CCTCGTGAGCTACCAAACTG	AGCCTTCCAAGCTAACGATG	55.0	50.0	58.3	58.0	166 bp	1.0	78.4
trnfM-CAU-trnG-UCC_p4	CCTCGTGAGCTACCAAACTG	CCTAGCCTTCCAAGCTAACG	55.0	55.0	58.3	58.1	169 bp	1.0	78.2
trnfM-CAU-trnG-UCC_p5	CCTCAAGGTTATGAGCCTCG	CCAACGTCGACTATAACCCC	55.0	55.0	57.8	57.8	202 bp	1.0	77.2