Hotspot

rps16

LSC · 5113-6273 · 1161 bp

Back to Hilliella

Pi 0.0255

MarkerSeek score 61.90

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1161
variable_sites: 29
pi: 0.0255
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	GTTGAGTTAGCAACCCCCAA	TTTGTGTGTGATGCGGGTAA	50.0	45.0	58.0	58.0	2149 bp	0.5	59.7
rps16_p2	CATGTGTGCGTCAGGGATAA	TTTGTGTGTGATGCGGGTAA	50.0	45.0	58.0	58.0	2056 bp	0.5	59.6
rps16_p3	TGTGCGTCAGGGATAAATGG	TTTGTGTGTGATGCGGGTAA	50.0	45.0	58.0	58.0	2052 bp	0.5	59.5
rps16_p4	GATGGAAGCGAAAGAGGAGG	TTTGTGTGTGATGCGGGTAA	55.0	45.0	58.1	58.0	1531 bp	0.5	59.3
rps16_p5	GTTGAGTTAGCAACCCCCAA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	2337 bp	0.5	59.3