Hotspot

trnL-CUA-ccsA

SSC · 114878-114993 · 116 bp

Back to Hilliella

Pi 0.0708

MarkerSeek score 76.60

Primer available yes

Label trnL-CUA-ccsA

Feature Metadata

label_name: trnL-CUA-ccsA
feature_type: igs
region: SSC
length_bp: 116
variable_sites: 8
pi: 0.0708
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CUA-ccsA_p1	TAGACACGCTGCTCTTAGGA	TCAGTACAATTGAAACGACCGA	50.0	40.9	57.9	58.0	241-244 bp	1.0	66.5
trnL-CUA-ccsA_p2	GTTTTGAACAAGTTGCCGCT	TCAGTACAATTGAAACGACCGA	45.0	40.9	58.1	58.0	274-277 bp	1.0	66.4
trnL-CUA-ccsA_p3	AAGTTGCCGCTATGGTGAAA	TCAGTACAATTGAAACGACCGA	45.0	40.9	58.1	58.0	265-268 bp	1.0	66.4
trnL-CUA-ccsA_p4	GTTTTGAACAAGTTGCCGCT	GTCAGTACAATTGAAACGACCG	45.0	45.5	58.1	58.0	275-278 bp	1.0	66.2
trnL-CUA-ccsA_p5	AAGTTGCCGCTATGGTGAAA	GTCAGTACAATTGAAACGACCG	45.0	45.5	58.1	58.0	266-269 bp	1.0	66.1