Hotspot

ycf1-rpl32

SSC · 110624-113697 · 3074 bp

Back to Hilliella

Pi 0.0194
MarkerSeek score 52.90
Primer available yes
Label ycf1-rpl32

Feature Metadata

label_name
ycf1-rpl32
feature_type
igs
region
SSC
length_bp
3074
variable_sites
59
pi
0.0194
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-rpl32_p1 GTCCACATACGTCGAAGGTT TCAGTGGTATAATTGGATGTCA 50.0 36.4 57.6 54.6 958-971 bp 1.0 45.1
ycf1-rpl32_p2 AGTCCACATACGTCGAAGGT TCAGTGGTATAATTGGATGTCA 50.0 36.4 58.5 54.6 959-972 bp 1.0 45.1
ycf1-rpl32_p3 GTCCACATACGTCGAAGGTT TCAGTGGTATAATTGGATGTCAA 50.0 34.8 57.6 55.4 958-971 bp 1.0 45.1
ycf1-rpl32_p4 GTCCACATACGTCGAAGGTT TTCAGTGGTATAATTGGATGTCA 50.0 34.8 57.6 55.4 959-972 bp 1.0 45.1
ycf1-rpl32_p5 GTCCACATACGTCGAAGGTT GTGGTATAATTGGATGTCAAAGACA 50.0 36.0 57.6 57.4 955-968 bp 1.0 45.1