Hotspot

psbB-psbT

LSC · 73887-74074 · 188 bp

Back to Hilliella

Pi 0.0535

MarkerSeek score 69.90

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 188
variable_sites: 10
pi: 0.0535
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	CCAACTACAAAAAGACAAGCAGT	TGTATAAACCAATGCTTCCA	39.1	35.0	57.8	52.4	235-236 bp	1.0	43.7
psbB-psbT_p2	CCAACTACAAAAAGACAAGCAGT	AGAAATGTATAAACCAATGCTTCCA	39.1	32.0	57.8	57.3	240-241 bp	1.0	43.7
psbB-psbT_p3	TCCAACTACAAAAAGACAAGCAG	TGTATAAACCAATGCTTCCA	39.1	35.0	57.5	52.4	236-237 bp	1.0	43.7
psbB-psbT_p4	CCAACTACAAAAAGACAAGCAGT	TCCCTAAAGTCGATACTAAGAGA	39.1	39.1	57.8	55.4	260-261 bp	1.0	43.4
psbB-psbT_p5	TCCAACTACAAAAAGACAAGCAG	TCCCTAAAGTCGATACTAAGAGA	39.1	39.1	57.5	55.4	261-262 bp	1.0	43.4