Hotspot

petG-trnW-UGG

LSC · 66098-66230 · 133 bp

Back to Hilliella

Pi 0.0391

MarkerSeek score 71.40

Primer available yes

Label petG-trnW-UGG

Feature Metadata

label_name: petG-trnW-UGG
feature_type: igs
region: LSC
length_bp: 133
variable_sites: 5
pi: 0.0391
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-UGG_p1	ACAGACGTGGTGATCAGTTG	AGAACGTGGGTCTCCAAAAC	50.0	50.0	57.8	58.0	210-215 bp	1.0	82.2
petG-trnW-UGG_p2	CGTGGTGATCAGTTGGACTT	AGAACGTGGGTCTCCAAAAC	50.0	50.0	57.8	58.0	205-210 bp	1.0	82.2
petG-trnW-UGG_p3	ACAGACGTGGTGATCAGTTG	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.8	58.0	232-237 bp	1.0	82.0
petG-trnW-UGG_p4	CGTGGTGATCAGTTGGACTT	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.8	58.0	227-232 bp	1.0	82.0
petG-trnW-UGG_p5	ACAGACGTGGTGATCAGTTG	CGCTCTTAGTTCAGTTCGGT	50.0	50.0	57.8	57.7	230-235 bp	1.0	79.4