Hotspot

ycf4-cemA

LSC · 60201-60678 · 478 bp

Back to Hilliella

Pi 0.0278

MarkerSeek score 70.60

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 478
variable_sites: 13
pi: 0.0278
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCTTACTTCTTGCGTGT	AGAGAGATCAACCAGGGCAA	45.0	50.0	58.0	58.3	591-600 bp	1.0	80.1
ycf4-cemA_p2	AGGTCAAGGGGCTATTCCTT	AGAGAGATCAACCAGGGCAA	50.0	50.0	58.0	58.3	667-676 bp	1.0	79.9
ycf4-cemA_p3	TTGGCTTACTTCTTGCGTGT	TCAACCAGGGCAAAAAGACT	45.0	45.0	58.0	57.5	584-593 bp	1.0	79.2
ycf4-cemA_p4	AGGTCAAGGGGCTATTCCTT	TCAACCAGGGCAAAAAGACT	50.0	45.0	58.0	57.5	660-669 bp	1.0	79.0
ycf4-cemA_p5	AGGTCAAGGGGCTATTCCTT	GAGAGATCAACCAGGGCAAA	50.0	50.0	58.0	57.5	666-675 bp	1.0	78.7