Hotspot

atpB-rbcL

LSC · 54052-54850 · 799 bp

Back to Hilliella

Pi 0.0205
MarkerSeek score 72.80
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
799
variable_sites
16
pi
0.0205
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 CATCCAGTACCGGACCAATG CACCAGCTTTGAATCCAACAC 55.0 47.6 58.0 58.0 934-945 bp 1.0 74.5
atpB-rbcL_p2 CGAAACCTCTGGATCCGAAG CACCAGCTTTGAATCCAACAC 55.0 47.6 58.1 58.0 876-887 bp 1.0 74.5
atpB-rbcL_p3 CATCCAGTACCGGACCAATG ACCAGCTTTGAATCCAACACT 55.0 42.9 58.0 58.1 933-944 bp 1.0 74.2
atpB-rbcL_p4 CGAAACCTCTGGATCCGAAG ACCAGCTTTGAATCCAACACT 55.0 42.9 58.1 58.1 875-886 bp 1.0 74.2
atpB-rbcL_p5 TCCAGTACCGGACCAATGAT CACCAGCTTTGAATCCAACAC 50.0 47.6 58.1 58.0 932-943 bp 1.0 73.8