Hotspot

trnS-UCC-rps4

LSC · 44965-45252 · 288 bp

Back to Hilliella

Pi 0.0459

MarkerSeek score 80.50

Primer available yes

Label trnS-UCC-rps4

Feature Metadata

label_name: trnS-UCC-rps4
feature_type: igs
region: LSC
length_bp: 288
variable_sites: 13
pi: 0.0459
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UCC-rps4_p1	GAGTGGTTGAAGGCGTAACA	AGGAAATGCGTCGGTTTGAA	50.0	45.0	58.1	58.4	424-429 bp	1.0	79.2
trnS-UCC-rps4_p2	GAGTGGTTGAAGGCGTAACA	GGAAATGCGTCGGTTTGAAA	50.0	45.0	58.1	57.6	423-428 bp	1.0	79.1
trnS-UCC-rps4_p3	GAGTGGTTGAAGGCGTAACA	TAGGAAATGCGTCGGTTTGA	50.0	45.0	58.1	57.5	425-430 bp	1.0	78.7
trnS-UCC-rps4_p4	TCCTGATGTCGGAGAGATGG	AGGAAATGCGTCGGTTTGAA	55.0	45.0	58.3	58.4	446-451 bp	1.0	77.6
trnS-UCC-rps4_p5	TCCTGATGTCGGAGAGATGG	GGAAATGCGTCGGTTTGAAA	55.0	45.0	58.3	57.6	445-450 bp	1.0	77.5