Hotspot

psaA-ycf3

LSC · 41903-42624 · 722 bp

Back to Hilliella

Pi 0.0364

MarkerSeek score 67.10

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 722
variable_sites: 26
pi: 0.0364
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CTTCTGGTTCCGGCGAAC	CACGAGGCGCTTCGAATAA	61.1	52.6	58.4	58.3	769-774 bp	1.0	53.2
psaA-ycf3_p2	CTTCTGGTTCCGGCGAAC	ATCACGAGGCGCTTCGAATA	61.1	50.0	58.4	59.6	771-776 bp	1.0	51.0
psaA-ycf3_p3	ACTTCTGGTTCCGGCGAA	CACGAGGCGCTTCGAATAA	55.6	52.6	58.9	58.3	770-775 bp	1.0	49.9
psaA-ycf3_p4	CTTCTGGTTCCGGCGAAC	TCACGAGGCGCTTCGAATAA	61.1	50.0	58.4	59.8	770-775 bp	1.0	49.3
psaA-ycf3_p5	ACTTCTGGTTCCGGCGAA	ATCACGAGGCGCTTCGAATA	55.6	50.0	58.9	59.6	772-777 bp	1.0	47.6