Hotspot

psbZ-trnG-GGC

LSC · 36046-36536 · 491 bp

Back to Hilliella

Pi 0.0215

MarkerSeek score 69.60

Primer available yes

Label psbZ-trnG-GGC

Feature Metadata

label_name: psbZ-trnG-GGC
feature_type: igs
region: LSC
length_bp: 491
variable_sites: 10
pi: 0.0215
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GGC_p1	TCCTGATGGTTGGTCGAGTA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	631-641 bp	1.0	81.2
psbZ-trnG-GGC_p2	CCGTTGTATTTGCGTCTCCT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.3	57.8	647-657 bp	1.0	80.7
psbZ-trnG-GGC_p3	TTTGCGTCTCCTGATGGTTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.5	57.8	639-649 bp	1.0	79.2
psbZ-trnG-GGC_p4	ATTTGCGTCTCCTGATGGTT	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.5	57.8	640-650 bp	1.0	79.0
psbZ-trnG-GGC_p5	CGTTGTATTTGCGTCTCCTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.2	57.8	646-656 bp	1.0	76.6