Hotspot

trnC-UGC-petN

LSC · 27582-28192 · 611 bp

Back to Hilliella

Pi 0.02

MarkerSeek score 64.00

Primer available yes

Label trnC-UGC-petN

Feature Metadata

label_name: trnC-UGC-petN
feature_type: igs
region: LSC
length_bp: 611
variable_sites: 12
pi: 0.02
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-UGC-petN_p1	TTTCCCCAGTTCAAATCCGG	ACTTCTTCCCCACACTACGA	50.0	50.0	58.1	58.0	720-721 bp	1.0	81.7
trnC-UGC-petN_p2	GTTTCTATCGTTTTGGCGGC	ACTTCTTCCCCACACTACGA	50.0	50.0	58.1	58.0	776-778 bp	1.0	81.5
trnC-UGC-petN_p3	TTTCCCCAGTTCAAATCCGG	CACTTCTTCCCCACACTACG	50.0	55.0	58.1	57.9	721-722 bp	1.0	81.1
trnC-UGC-petN_p4	GTTTCTATCGTTTTGGCGGC	CACTTCTTCCCCACACTACG	50.0	55.0	58.1	57.9	777-779 bp	1.0	80.9
trnC-UGC-petN_p5	TGCAAATCCCTTTTTCCCCA	ACTTCTTCCCCACACTACGA	45.0	50.0	57.9	58.0	733 bp	0.5	59.1