Hotspot

trnS-AGC-trnG-GGA

LSC · 8035-8713 · 679 bp

Back to Hilliella

Pi 0.033

MarkerSeek score 71.30

Primer available yes

Label trnS-AGC-trnG-GGA

Feature Metadata

label_name: trnS-AGC-trnG-GGA
feature_type: igs
region: LSC
length_bp: 679
variable_sites: 21
pi: 0.033
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-AGC-trnG-GGA_p1	CCAACGCTTTAGTCCACTCA	AGGGGTTAAAGGAACGAATCA	50.0	42.9	57.8	56.9	738-757 bp	1.0	68.3
trnS-AGC-trnG-GGA_p2	CCAACGCTTTAGTCCACTCA	GGGGTTAAAGGAACGAATCAC	50.0	47.6	57.8	56.9	737-756 bp	1.0	68.0
trnS-AGC-trnG-GGA_p3	AACGCTTTAGTCCACTCAGC	AGGGGTTAAAGGAACGAATCA	50.0	42.9	58.2	56.9	736-755 bp	1.0	68.0
trnS-AGC-trnG-GGA_p4	AACGCTTTAGTCCACTCAGC	GGGGTTAAAGGAACGAATCAC	50.0	47.6	58.2	56.9	735-754 bp	1.0	67.7
trnS-AGC-trnG-GGA_p5	CCAACGCTTTAGTCCACTCA	AGGGGTTAAAGGAACGAATCAC	50.0	45.5	57.8	58.3	738-757 bp	1.0	66.4