Hotspot

trnK-AAA-rps16

LSC · 4426-5112 · 687 bp

Back to Hilliella

Pi 0.0312
MarkerSeek score 63.80
Primer available yes
Label trnK-AAA-rps16

Feature Metadata

label_name
trnK-AAA-rps16
feature_type
igs
region
LSC
length_bp
687
variable_sites
21
pi
0.0312
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-AAA-rps16_p1 CCTTTCAGGATCAGTCGTGG CCCAATGAGCCGTTTATCGA 55.0 50.0 58.0 58.1 1050-1072 bp 1.0 82.0
trnK-AAA-rps16_p2 CCTTTCAGGATCAGTCGTGG TCCCAATGAGCCGTTTATCG 55.0 50.0 58.0 58.1 1051-1073 bp 1.0 82.0
trnK-AAA-rps16_p3 TCGACACCGAAAACAAGGTT CCCAATGAGCCGTTTATCGA 45.0 50.0 58.0 58.1 1145-1165 bp 1.0 82.0
trnK-AAA-rps16_p4 TCGACACCGAAAACAAGGTT TCCCAATGAGCCGTTTATCG 45.0 50.0 58.0 58.1 1146-1166 bp 1.0 82.0
trnK-AAA-rps16_p5 CCTTTCAGGATCAGTCGTGG CGTTTCTAGGGGGAGGGTAT 55.0 55.0 58.0 57.9 1087 bp 0.5 59.1