Hotspot

trnH-CAC-psbA

LSC · 180-458 · 279 bp

Back to Hilliella

Pi 0.0903

MarkerSeek score 73.80

Primer available yes

Label trnH-CAC-psbA

Feature Metadata

label_name: trnH-CAC-psbA
feature_type: igs
region: LSC
length_bp: 279
variable_sites: 25
pi: 0.0903
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-CAC-psbA_p1	CTGCCTTAATCCACTTGGCT	TGAGGCTCCATCTACAAATGG	50.0	47.6	57.9	57.4	332-336 bp	1.0	72.9
trnH-CAC-psbA_p2	ACTGCCTTAATCCACTTGGC	TGAGGCTCCATCTACAAATGG	50.0	47.6	58.2	57.4	333-337 bp	1.0	72.6
trnH-CAC-psbA_p3	AATCCACTTGGCTACATCCG	TGAGGCTCCATCTACAAATGG	50.0	47.6	57.7	57.4	325-329 bp	1.0	71.4
trnH-CAC-psbA_p4	CTGCCTTAATCCACTTGGCT	TTGAGGCTCCATCTACAAATGG	50.0	45.5	57.9	58.1	333-337 bp	1.0	68.5
trnH-CAC-psbA_p5	ATAACTTGGTCTCGGGCATC	TGAGGCTCCATCTACAAATGG	50.0	47.6	57.4	57.4	401 bp	0.5	42.5