Hotspot

ndhF-rpl32

SSC · 103521-104254 · 734 bp

Back to Hilaria

Pi 0.0193
MarkerSeek score 58.60
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
734
variable_sites
14
pi
0.0193
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGATTACCCAGGCATATTGA TGACATCGAAGTACGCTTTT 45.0 40.0 54.5 55.4 801-968 bp 1.0 42.1
ndhF-rpl32_p2 GGATTACCCAGGCATATTGA TTGACATCGAAGTACGCTTT 45.0 40.0 54.5 55.4 802-969 bp 1.0 42.1
ndhF-rpl32_p3 GGATTACCCAGGCATATTGA TTTGACATCGAAGTACGCTT 45.0 40.0 54.5 55.4 803-970 bp 1.0 42.1
ndhF-rpl32_p4 GGATTACCCAGGCATATTGA TTTTGACATCGAAGTACGCT 45.0 40.0 54.5 55.4 804-971 bp 1.0 42.1
ndhF-rpl32_p5 GGATTACCCAGGCATATTGA TGACATCGAAGTACGCTTTTT 45.0 38.1 54.5 56.2 801-968 bp 1.0 42.1