Hotspot

psaJ-rpl33

LSC · 64533-64949 · 417 bp

Back to Hilaria

Pi 0.024
MarkerSeek score 55.80
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
417
variable_sites
10
pi
0.024
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 GATGCTTTGTCATTCCCTTT ACCCTTTCGAACACAACTGA 40.0 45.0 54.1 57.3 510-518 bp 1.0 44.9
psaJ-rpl33_p2 GATGCTTTGTCATTCCCTTTT ACCCTTTCGAACACAACTGA 38.1 45.0 55.0 57.3 510-518 bp 1.0 43.8
psaJ-rpl33_p3 TGCTTTGTCATTCCCTTTTT ACCCTTTCGAACACAACTGA 35.0 45.0 53.8 57.3 508-516 bp 1.0 42.7
psaJ-rpl33_p4 GATGCTTTGTCATTCCCTTT TGGTACCCTTTCGAACACAA 40.0 45.0 54.1 57.0 514-522 bp 1.0 42.5
psaJ-rpl33_p5 GATGCTTTGTCATTCCCTTT TTGGTACCCTTTCGAACACA 40.0 45.0 54.1 57.0 515-523 bp 1.0 42.5