Hotspot

trnP-UGG-psaJ

LSC · 64001-64403 · 403 bp

Back to Hilaria

Pi 0.0126

MarkerSeek score 55.90

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 403
variable_sites: 5
pi: 0.0126
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	AAAACAAACGCGCTACCAAG	AAACCTGCTAAAGCCCCAAA	45.0	45.0	57.9	57.9	507-513 bp	1.0	80.0
trnP-UGG-psaJ_p2	CAAAACAAACGCGCTACCAA	AAACCTGCTAAAGCCCCAAA	45.0	45.0	58.2	57.9	508-514 bp	1.0	79.6
trnP-UGG-psaJ_p3	TTGTACCCAAAACAAACGCG	AAACCTGCTAAAGCCCCAAA	45.0	45.0	57.8	57.9	515-521 bp	1.0	79.4
trnP-UGG-psaJ_p4	CCGCTACTGCCCATAAAGAA	AAACCTGCTAAAGCCCCAAA	50.0	45.0	57.7	57.9	581-587 bp	1.0	78.1
trnP-UGG-psaJ_p5	TACCCAAAACAAACGCGCTA	AAACCTGCTAAAGCCCCAAA	45.0	45.0	58.4	57.9	512-518 bp	1.0	77.7