Hotspot

petG-trnW-CCA

LSC · 63597-63727 · 131 bp

Back to Hilaria

Pi 0.0846
MarkerSeek score 77.10
Primer available yes
Label petG-trnW-CCA

Feature Metadata

label_name
petG-trnW-CCA
feature_type
igs
region
LSC
length_bp
131
variable_sites
11
pi
0.0846
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petG-trnW-CCA_p1 GCGTATTTGCAATACAGGCG CAAAACCCGATGTCGTAGGT 50.0 50.0 58.3 57.9 212-217 bp 1.0 81.7
petG-trnW-CCA_p2 TCGTGACTGCGTATTTGCAA CAAAACCCGATGTCGTAGGT 45.0 50.0 58.5 57.9 220-225 bp 1.0 79.6
petG-trnW-CCA_p3 TTCGTGACTGCGTATTTGCA CAAAACCCGATGTCGTAGGT 45.0 50.0 58.5 57.9 221-226 bp 1.0 79.6
petG-trnW-CCA_p4 GCGTATTTGCAATACAGGCG CCCGATGTCGTAGGTTCAAA 50.0 50.0 58.3 57.6 207-212 bp 1.0 79.5
petG-trnW-CCA_p5 CGTGACTGCGTATTTGCAAT CAAAACCCGATGTCGTAGGT 45.0 50.0 57.5 57.9 219-224 bp 1.0 79.4