Hotspot

petA-psbJ

LSC · 60240-61147 · 908 bp

Back to Hilaria

Pi 0.0145

MarkerSeek score 68.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 908
variable_sites: 13
pi: 0.0145
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTCCAAGGCCTTTTGTTCT	ACGGTTCGTATTCTGGATTGG	50.0	47.6	58.7	58.1	1039-1052 bp	1.0	67.8
petA-psbJ_p2	GATCCATTACGCGTCCAAGG	ACGGTTCGTATTCTGGATTGG	55.0	47.6	58.8	58.1	1050-1063 bp	1.0	67.0
petA-psbJ_p3	CGTCCAAGGCCTTTTGTTCT	GGTTCGTATTCTGGATTGGGT	50.0	47.6	58.7	57.7	1037-1050 bp	1.0	66.4
petA-psbJ_p4	CGTCCAAGGCCTTTTGTTCT	GCTGATAGGTACTGTAACCGG	50.0	52.4	58.7	57.6	1095-1108 bp	1.0	65.6
petA-psbJ_p5	CGTCCAAGGCCTTTTGTTCT	GGCTGATAGGTACTGTAACCG	50.0	52.4	58.7	57.6	1096-1109 bp	1.0	65.6