Hotspot

atpA-trnR-UCU

LSC · 36352-36491 · 140 bp

Back to Hilaria

Pi 0.0301

MarkerSeek score 68.50

Primer available yes

Label atpA-trnR-UCU

Feature Metadata

label_name: atpA-trnR-UCU
feature_type: igs
region: LSC
length_bp: 140
variable_sites: 4
pi: 0.0301
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-trnR-UCU_p1	TGAACGGTTTTCCCTTCAGG	GGTTCAAATCCTATTGGACGC	50.0	47.6	58.0	57.3	193-196 bp	1.0	70.8
atpA-trnR-UCU_p2	AGCTTGAACGGTTTTCCCTT	GGTTCAAATCCTATTGGACGC	45.0	47.6	57.9	57.3	197-200 bp	1.0	70.4
atpA-trnR-UCU_p3	AGGAACAGCTTGAACGGTTT	GGTTCAAATCCTATTGGACGC	45.0	47.6	57.9	57.3	203-206 bp	1.0	70.3
atpA-trnR-UCU_p4	TAGCAAGACATTCACCGAGC	GGTTCAAATCCTATTGGACGC	50.0	47.6	58.0	57.3	253-256 bp	1.0	69.8
atpA-trnR-UCU_p5	GAACGGTTTTCCCTTCAGGA	GGTTCAAATCCTATTGGACGC	50.0	47.6	57.7	57.3	192-195 bp	1.0	68.9