Hotspot

atpH-atpF

LSC · 32903-33345 · 443 bp

Back to Hilaria

Pi 0.0137

MarkerSeek score 63.20

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: LSC
length_bp: 443
variable_sites: 6
pi: 0.0137
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	TTATGGACTGGTTGTGGCAC	CGAATGGCCAGTGAGCTAAA	50.0	50.0	58.1	58.3	544 bp	1.0	78.9
atpH-atpF_p2	TGTGGCACTAGCGCTTTTAT	CGAATGGCCAGTGAGCTAAA	45.0	50.0	57.9	58.3	532 bp	1.0	78.8
atpH-atpF_p3	TTGTGGCACTAGCGCTTTTA	CGAATGGCCAGTGAGCTAAA	45.0	50.0	58.1	58.3	533 bp	1.0	78.8
atpH-atpF_p4	TTATGGACTGGTTGTGGCAC	ATTAAGCCCGAAACTCCCAG	50.0	50.0	58.1	57.6	564 bp	1.0	77.6
atpH-atpF_p5	TGTGGCACTAGCGCTTTTAT	ATTAAGCCCGAAACTCCCAG	45.0	50.0	57.9	57.6	552 bp	1.0	77.5