Hotspot

petN-trnC-GCA

LSC · 17808-18748 · 941 bp

Back to Hilaria

Pi 0.0175

MarkerSeek score 67.00

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 941
variable_sites: 16
pi: 0.0175
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGTCCACTTCTCCCCCATAC	ATTGGATTTTTGGCGGCATG	55.0	45.0	58.1	58.0	1091-1109 bp	1.0	82.1
petN-trnC-GCA_p2	GTCCACTTCTCCCCCATACT	ATTGGATTTTTGGCGGCATG	55.0	45.0	58.1	58.0	1090-1108 bp	1.0	82.1
petN-trnC-GCA_p3	AGCCCAAGCGAGACTTACTA	ATTGGATTTTTGGCGGCATG	50.0	45.0	58.2	58.0	1032-1050 bp	1.0	82.0
petN-trnC-GCA_p4	GAGTCCACTTCTCCCCCATA	ATTGGATTTTTGGCGGCATG	55.0	45.0	57.8	58.0	1092-1110 bp	1.0	81.6
petN-trnC-GCA_p5	CTAGAGTCCACTTCTCCCCC	ATTGGATTTTTGGCGGCATG	60.0	45.0	58.2	58.0	1095-1113 bp	1.0	81.3