Hotspot

rps16

LSC · 5201-6319 · 1119 bp

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Pi 0.00366

MarkerSeek score 53.70

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1119
variable_sites: 13
pi: 0.00366
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AGAAATCACTATCCTACCCTTAGTT	TCTCTGTACCGACTGAACCA	36.0	50.0	56.8	57.7	1237-1244 bp	1.0	41.2
rps16_p2	AGAAATCACTATCCTACCCTTAGT	TCTCTGTACCGACTGAACCA	37.5	50.0	56.1	57.7	1237-1244 bp	0.9	37.1
rps16_p3	AGAAATCACTATCCTACCCT	TCTCTGTACCGACTGAACCA	40.0	50.0	52.0	57.7	1237-1244 bp	0.9	37.1
rps16_p4	AGAAATCACTATCCTACCCTT	TCTCTGTACCGACTGAACCA	38.1	50.0	53.0	57.7	1237-1244 bp	0.9	37.1
rps16_p5	AGAAATCACTATCCTACCCTTAGT	GTCTCTGTACCGACTGAACC	37.5	55.0	56.1	57.4	1238-1245 bp	0.9	37.1