Hotspot

rpl16

LSC · 81459-82897 · 1439 bp

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Pi 0.00272

MarkerSeek score 54.70

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1439
variable_sites: 13
pi: 0.00272
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	AAACCTGCACCCGCTATTTT	TGAACAATTATAATTCTATAGGGTTGA	45.0	25.9	58.4	54.8	1542-1576 bp	1.0	41.4
rpl16_p2	AACCTGCACCCGCTATTTTT	TGAACAATTATAATTCTATAGGGTTGA	45.0	25.9	58.4	54.8	1541-1575 bp	1.0	41.4
rpl16_p3	AAACCTGCACCCGCTATTTT	TGAACAATTATAATTCTATAGGGTTG	45.0	26.9	58.4	53.5	1542-1576 bp	1.0	41.4
rpl16_p4	AACCTGCACCCGCTATTTTT	TGAACAATTATAATTCTATAGGGTTG	45.0	26.9	58.4	53.5	1541-1575 bp	1.0	41.4
rpl16_p5	GAAACCTGCACCCGCTATTT	TGAACAATTATAATTCTATAGGGTTGA	50.0	25.9	58.8	54.8	1543-1577 bp	1.0	41.4