Hotspot

rps15-ycf1

SSC · 122957-123368 · 412 bp

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Pi 0.00809

MarkerSeek score 45.60

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 412
variable_sites: 11
pi: 0.00809
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	ACTTGAAATTCAACAGATCCTCT	AATAATGGGAGCCGCTTGAG	34.8	50.0	55.8	58.0	543-549 bp	1.0	41.6
rps15-ycf1_p2	CAGATCCTCTGTTTTCTTTGT	AATAATGGGAGCCGCTTGAG	38.1	50.0	53.7	58.0	530-536 bp	1.0	41.6
rps15-ycf1_p3	CAGATCCTCTGTTTTCTTTGTT	AATAATGGGAGCCGCTTGAG	36.4	50.0	54.6	58.0	530-536 bp	1.0	41.6
rps15-ycf1_p4	ACTTGAAATTCAACAGATCCTCT	CAATAATGGGAGCCGCTTGA	34.8	50.0	55.8	58.3	544-550 bp	1.0	41.6
rps15-ycf1_p5	CAGATCCTCTGTTTTCTTTGT	CAATAATGGGAGCCGCTTGA	38.1	50.0	53.7	58.3	531-537 bp	1.0	41.6