Hotspot

rpl20-rps12

LSC · 69182-69928 · 747 bp

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Pi 0.00411

MarkerSeek score 54.80

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 747
variable_sites: 12
pi: 0.00411
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCTCCGAGCGATATATCCCC	CAGCGTCGAGGAACATGTAC	55.0	55.0	57.9	58.7	776-814 bp	1.0	78.8
rpl20-rps12_p2	TCTCCGAGCGATATATCCCC	AGCGTCGAGGAACATGTACT	55.0	50.0	57.9	58.8	775-813 bp	1.0	78.0
rpl20-rps12_p3	TCTCCGAGCGATATATCCCC	TCAGCGTCGAGGAACATGTA	55.0	50.0	57.9	58.8	777-815 bp	1.0	78.0
rpl20-rps12_p4	TCTCCGAGCGATATATCCCC	GCGTCGAGGAACATGTACTA	55.0	50.0	57.9	57.2	774-812 bp	1.0	77.9
rpl20-rps12_p5	TTCTACGTCTCCGAGCGATA	CAGCGTCGAGGAACATGTAC	50.0	55.0	57.8	58.7	783-821 bp	1.0	77.3