Hotspot

ndhC-trnV-UAC

LSC · 50386-51601 · 1216 bp

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Pi 0.00504
MarkerSeek score 56.60
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1216
variable_sites
18
pi
0.00504
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.97
markerseek_score
56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCCTTTGCTACTCGGAGCTA CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.9 58.1 961-1366 bp 1.0 83.2
ndhC-trnV-UAC_p2 ACTCGGAGCTAAAAATCCCG CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.7 58.1 952-1357 bp 1.0 81.9
ndhC-trnV-UAC_p3 TTCTCCGGTCCTTTGCTACT CCGAGAAAGTCTACGGTTCG 50.0 55.0 58.4 58.1 969-1374 bp 1.0 81.5
ndhC-trnV-UAC_p4 TTTCTCCGGTCCTTTGCTAC CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.5 58.1 970-1375 bp 1.0 80.6
ndhC-trnV-UAC_p5 CTCGGAGCTAAAAATCCCGA CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.4 58.1 951-1356 bp 1.0 79.6