Hotspot

trnC-GCA-petN

LSC · 9476-10325 · 850 bp

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Pi 0.00721
MarkerSeek score 47.70
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
850
variable_sites
18
pi
0.00721
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
47.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG AGCCCAAGCGAGACTTACTA 50.0 50.0 58.1 58.2 891-906 bp 1.0 81.2
trnC-GCA-petN_p2 GGGGGACTGCAAATCCTTTT AGCCCAAGCGAGACTTACTA 50.0 50.0 58.4 58.2 909-924 bp 1.0 78.9
trnC-GCA-petN_p3 TAGTGTATCGTTTTGGCGGC AGCCCAAGCGAGACTTACTA 50.0 50.0 58.6 58.2 947-962 bp 1.0 76.5
trnC-GCA-petN_p4 TTTCCCCAGTTCAAATCCGG GCCCAAGCGAGACTTACTAT 50.0 50.0 58.1 57.1 890-905 bp 1.0 75.2
trnC-GCA-petN_p5 TTTCCCCAGTTCAAATCCGG ATTAAAGCAGCCCAAGCGAG 50.0 50.0 58.1 58.9 899-914 bp 1.0 75.1