Hotspot

ccsA-ndhD

SSC · 123064-123369 · 306 bp

Back to Hibiscus

Pi 0.0602

MarkerSeek score 77.70

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 306
variable_sites: 45
pi: 0.0602
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GTAAATTCCGCACTTGTGGC	TTTTTGATTCTGGCCCACGA	50.0	45.0	58.0	58.0	568-614 bp	1.0	89.9
ccsA-ndhD_p2	TTGGACCGTATTCGCGATTT	TTTTTGATTCTGGCCCACGA	45.0	45.0	58.0	58.0	623-669 bp	1.0	89.3
ccsA-ndhD_p3	TAAATTCCGCACTTGTGGCT	TTTTTGATTCTGGCCCACGA	45.0	45.0	58.1	58.0	567-613 bp	1.0	89.3
ccsA-ndhD_p4	TTACTTGGACCGTATTCGCG	TTTTTGATTCTGGCCCACGA	50.0	45.0	58.1	58.0	627-673 bp	1.0	88.7
ccsA-ndhD_p5	TTGGACCGTATTCGCGATTT	TGGCCCACGAGAGTTATTTG	45.0	50.0	58.0	57.9	613-659 bp	1.0	88.7