Hotspot

ndhF-rpl32

SSC · 119395-120570 · 1176 bp

Back to Hibiscus

Pi 0.0769

MarkerSeek score 76.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1176
variable_sites: 196
pi: 0.0769
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGGAAGTGGCACAAAAGGTA	GGGGTTTTCTCAAGTGGGTT	50.0	50.0	58.0	57.9	1581-1841 bp	0.555556	73.5
ndhF-rpl32_p2	GCCCACATACGACGAAGATT	GGGGTTTTCTCAAGTGGGTT	50.0	50.0	58.1	57.9	1652-1912 bp	0.555556	73.1
ndhF-rpl32_p3	GGGAAGTGGCACAAAAGGTA	TGAAAAAGCTTTCAACGCCG	50.0	45.0	58.0	58.2	1001-1343 bp	0.555556	72.8
ndhF-rpl32_p4	GCCCACATACGACGAAGATT	TGAAAAAGCTTTCAACGCCG	50.0	45.0	58.1	58.2	1072-1414 bp	0.555556	72.4
ndhF-rpl32_p5	AAAAGCCCACATACGACGAA	GGGGTTTTCTCAAGTGGGTT	45.0	50.0	57.8	57.9	1656-1916 bp	0.555556	72.2