Hotspot

trnN-GUU-ndhF

IRb · 115573-117177 · 1605 bp

Back to Hibiscus

Pi 0.0816

MarkerSeek score 77.40

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 1605
variable_sites: 263
pi: 0.0816
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	GGCAGAATAGCAGAGCAAGT	ATATGTAGGGGGAAGTCGCA	50.0	50.0	58.0	57.9	1196-2174 bp	1.0	90.9
trnN-GUU-ndhF_p2	GGCAGAATAGCAGAGCAAGT	TATGTAGGGGGAAGTCGCAT	50.0	50.0	58.0	57.9	1195-2173 bp	1.0	90.9
trnN-GUU-ndhF_p3	GGGTTACCTGCTCTTAACGG	ATATGTAGGGGGAAGTCGCA	55.0	50.0	58.0	57.9	888-1866 bp	0.777778	82.2
trnN-GUU-ndhF_p4	GGGTTACCTGCTCTTAACGG	TATGTAGGGGGAAGTCGCAT	55.0	50.0	58.0	57.9	887-1865 bp	0.777778	82.2
trnN-GUU-ndhF_p5	ACGGTCAAAGCGAACTCTTT	TATGTAGGGGGAAGTCGCAT	45.0	50.0	58.1	57.9	871-1849 bp	0.777778	81.9