Hotspot

rpl33-rps18

LSC · 73020-73492 · 473 bp

Back to Hibiscus

Pi 0.0458
MarkerSeek score 73.40
Primer available yes
Label rpl33-rps18

Feature Metadata

label_name
rpl33-rps18
feature_type
igs
region
LSC
length_bp
473
variable_sites
47
pi
0.0458
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.81
markerseek_score
73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-rps18_p1 TACGCCTAGTCGATTGGAGT GGGTTCCCTCGAGAATGAAC 50.0 55.0 58.0 58.0 916-1154 bp 0.888889 84.1
rpl33-rps18_p2 TACGCCTAGTCGATTGGAGT AGGGTTCCCTCGAGAATGAA 50.0 50.0 58.0 58.0 917-1155 bp 0.888889 83.9
rpl33-rps18_p3 TGTGTTCGAAACGGCCTTAA GGGTTCCCTCGAGAATGAAC 45.0 55.0 58.0 58.0 1147-1196 bp 0.222222 52.0
rpl33-rps18_p4 TTGTGTTCGAAACGGCCTTA GGGTTCCCTCGAGAATGAAC 45.0 55.0 58.0 58.0 1148-1197 bp 0.222222 52.0
rpl33-rps18_p5 TGTGTTCGAAACGGCCTTAA AGGGTTCCCTCGAGAATGAA 45.0 50.0 58.0 58.0 1148-1197 bp 0.222222 51.9