Hotspot

petA-psbJ

LSC · 68099-69183 · 1085 bp

Back to Hibiscus

Pi 0.0552

MarkerSeek score 80.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1085
variable_sites: 158
pi: 0.0552
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGAGAAGGTTCAAGTGTCCG	ATTCTGGATTGGGTTCGTCC	50.0	50.0	57.5	57.6	852-1244 bp	1.0	84.4
petA-psbJ_p2	GAGAAGGTTCAAGTGTCCGA	ATTCTGGATTGGGTTCGTCC	50.0	50.0	57.2	57.6	851-1243 bp	1.0	82.1
petA-psbJ_p3	GAGAAGGTTCAAGTGTCCGAA	ATTCTGGATTGGGTTCGTCC	47.6	50.0	57.9	57.6	851-1243 bp	1.0	79.8
petA-psbJ_p4	TTGAGAAGGTTCAAGTGTCCG	ATTCTGGATTGGGTTCGTCC	47.6	50.0	58.2	57.6	853-1245 bp	1.0	79.3
petA-psbJ_p5	AGAAGGTTCAAGTGTCCGAA	ATTCTGGATTGGGTTCGTCC	45.0	50.0	56.7	57.6	850-1242 bp	1.0	78.3