Hotspot

atpB-rbcL

LSC · 58828-59966 · 1139 bp

Back to Hibiscus

Pi 0.0391

MarkerSeek score 77.40

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 1139
variable_sites: 122
pi: 0.0391
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GCCGACTGGAACACTTAGAG	GGTAGCATCGCCCTTTGTAA	55.0	50.0	58.0	58.0	1588-1734 bp	1.0	90.2
atpB-rbcL_p2	GCCGACTGGAACACTTAGAG	GTACCCGCAGTAGCATTCAA	55.0	50.0	58.0	58.0	2069-2215 bp	1.0	89.4
atpB-rbcL_p3	CGGTTCATTCATCTGACCGT	GTACCCGCAGTAGCATTCAA	50.0	50.0	58.0	58.0	2486-2632 bp	1.0	89.0
atpB-rbcL_p4	AAAATTCGTCCTAGGGTCGC	GTACCCGCAGTAGCATTCAA	50.0	50.0	58.0	58.0	2142-2238 bp	0.555556	68.1
atpB-rbcL_p5	GCCGACTGGAACACTTAGAG	TTGCTCCTCTTCTCCAGGAA	55.0	50.0	58.0	58.0	1710-1738 bp	0.333333	58.0