Hotspot

trnT-UGU-trnL-UAA

LSC · 49717-50635 · 919 bp

Back to Hibiscus

Pi 0.0444
MarkerSeek score 74.60
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
919
variable_sites
98
pi
0.0444
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.91
markerseek_score
74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG GACTTTTTGGGGTTGGGGAT 55.0 50.0 57.9 58.0 1506-1843 bp 1.0 89.4
trnT-UGU-trnL-UAA_p2 GCGATGCTCTAACCTCTGAG GCTTCCATTGAGTCTCTGCA 55.0 50.0 57.9 57.9 1041-1412 bp 1.0 89.4
trnT-UGU-trnL-UAA_p3 GCGATGCTCTAACCTCTGAG GGTTGGGGATAGAGGGACTT 55.0 55.0 57.9 58.1 1496-1833 bp 1.0 88.7
trnT-UGU-trnL-UAA_p4 GGAATCGAACCGATGACCAT GACTTTTTGGGGTTGGGGAT 50.0 50.0 57.8 58.0 1538-1875 bp 1.0 88.4
trnT-UGU-trnL-UAA_p5 GCGATGCTCTAACCTCTGAG AAAAATACGGGTTCGGGTCC 55.0 50.0 57.9 58.2 1225-1384 bp 0.222222 50.7