Hotspot

psaA-pafI

LSC · 44630-45540 · 911 bp

Back to Hibiscus

Pi 0.0271

MarkerSeek score 73.20

Primer available yes

Label psaA-pafI

Feature Metadata

label_name: psaA-pafI
feature_type: igs
region: LSC
length_bp: 911
variable_sites: 65
pi: 0.0271
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-pafI_p1	TTTTACTTCTGGTTCCGGCG	TGGTTAAAGATCACGAGGCG	50.0	50.0	58.5	58.0	863-1042 bp	1.0	85.4
psaA-pafI_p2	TTTTACTTCTGGTTCCGGCG	GGTTAAAGATCACGAGGCGT	50.0	50.0	58.5	58.0	862-1041 bp	1.0	85.4
psaA-pafI_p3	TTTTACTTCTGGTTCCGGCG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.5	58.0	931-1110 bp	1.0	84.8
psaA-pafI_p4	TTTTACTTCTGGTTCCGGCG	GGAAACAGGCTATAGCGCTT	50.0	50.0	58.5	58.0	913-1092 bp	1.0	84.8
psaA-pafI_p5	TTTTACTTCTGGTTCCGGCG	AGCCGCTGAGTATTGGAAAC	50.0	50.0	58.5	58.3	927-1106 bp	1.0	83.0