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psbZ-trnG-GCC

LSC · 38644-39242 · 599 bp

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Pi 0.0524

MarkerSeek score 85.30

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 599
variable_sites: 86
pi: 0.0524
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 85.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TGGATTAGTTTTTCTAGTGGGT	CGTCTTCTCCTTGGCAAAGA	36.4	50.0	54.7	57.8	594-1049 bp	1.0	52.0
psbZ-trnG-GCC_p2	GGATTGGATTAGTTTTTCTAGTGGG	CGTCTTCTCCTTGGCAAAGA	40.0	50.0	57.7	57.8	598-1053 bp	1.0	52.0
psbZ-trnG-GCC_p3	AGTTTTTCTAGTGGGTATCCT	CGTCTTCTCCTTGGCAAAGA	38.1	50.0	53.6	57.8	588-1043 bp	1.0	52.0
psbZ-trnG-GCC_p4	TTGGATTAGTTTTTCTAGTGGGT	CGTCTTCTCCTTGGCAAAGA	34.8	50.0	55.5	57.8	595-1050 bp	1.0	52.0
psbZ-trnG-GCC_p5	AGTTTTTCTAGTGGGTATCCTT	CGTCTTCTCCTTGGCAAAGA	36.4	50.0	54.4	57.8	588-1043 bp	1.0	52.0