Hotspot

trnR-UCU-atpA

LSC · 10537-10695 · 159 bp

Back to Hibiscus

Pi 0.071

MarkerSeek score 78.70

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 159
variable_sites: 28
pi: 0.071
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	AGCCTTCCAAGCTAACGATG	CCAGGTCGTGAAGCTTATCC	50.0	55.0	58.0	58.1	1148-1244 bp	1.0	85.8
trnR-UCU-atpA_p2	AGCCTTCCAAGCTAACGATG	GGTCGTGAAGCTTATCCAGG	50.0	55.0	58.0	58.1	1145-1241 bp	1.0	85.8
trnR-UCU-atpA_p3	CCTAGCCTTCCAAGCTAACG	CCAGGTCGTGAAGCTTATCC	55.0	55.0	58.1	58.1	1151-1247 bp	1.0	85.6
trnR-UCU-atpA_p4	CCTAGCCTTCCAAGCTAACG	GGTCGTGAAGCTTATCCAGG	55.0	55.0	58.1	58.1	1148-1244 bp	1.0	85.5
trnR-UCU-atpA_p5	AGCCTTCCAAGCTAACGATG	GCTTTTGCGCAATTTGCTTC	50.0	45.0	58.0	58.1	809-905 bp	0.777778	78.0