Hotspot

rps16-trnQ-UUG

LSC · 6492-7075 · 584 bp

Back to Hibiscus

Pi 0.047

MarkerSeek score 85.50

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 584
variable_sites: 75
pi: 0.047
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 85.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CAGCCGATCATGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	579-693 bp	1.0	91.2
rps16-trnQ-UUG_p2	GCCGATCATGTCCTTCAAGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	577-691 bp	1.0	91.2
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	561-675 bp	1.0	90.3
rps16-trnQ-UUG_p4	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	572-686 bp	1.0	89.8
rps16-trnQ-UUG_p5	AGCCGATCATGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.7	58.1	578-692 bp	1.0	88.8