Hotspot

trnK-UUU-rps16

LSC · 4503-5352 · 850 bp

Back to Hibiscus

Pi 0.0491

MarkerSeek score 77.80

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 850
variable_sites: 115
pi: 0.0491
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	ATCCACCCCTTTCAGGATCA	CCCAATGAGCCGTTTATCGA	50.0	50.0	58.0	58.1	1058-1293 bp	1.0	91.5
trnK-UUU-rps16_p2	ATCCACCCCTTTCAGGATCA	TCCCAATGAGCCGTTTATCG	50.0	50.0	58.0	58.1	1059-1294 bp	1.0	91.5
trnK-UUU-rps16_p3	ATCCACCCCTTTCAGGATCA	AAAAGGAGCTCAACCCACAG	50.0	50.0	58.0	58.0	919-1154 bp	0.777778	82.8
trnK-UUU-rps16_p4	CCCTTTCAGGATCAGTCGTG	AAAAGGAGCTCAACCCACAG	55.0	50.0	58.0	58.0	913-1148 bp	0.777778	82.8
trnK-UUU-rps16_p5	CCTTTCAGGATCAGTCGTGG	AAAAGGAGCTCAACCCACAG	55.0	50.0	58.0	58.0	912-1147 bp	0.777778	82.8