Hotspot

ycf3

LSC · 45987-47894 · 1908 bp

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Pi 0.000489
MarkerSeek score 49.40
Primer available yes
Label ycf3

Feature Metadata

label_name
ycf3
feature_type
gene
region
LSC
length_bp
1908
variable_sites
2
pi
0.000489
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3_p1 TGACTGATAGGGATAACAAACA TGTGGGATCGTTTTCTCATGA 36.4 42.9 54.5 57.3 2076-2084 bp 1.0 40.4
ycf3_p2 TGACTGATAGGGATAACAAACAA TGTGGGATCGTTTTCTCATGA 34.8 42.9 55.3 57.3 2076-2084 bp 1.0 40.4
ycf3_p3 TTGACTGATAGGGATAACAAACA TGTGGGATCGTTTTCTCATGA 34.8 42.9 55.3 57.3 2077-2085 bp 1.0 40.4
ycf3_p4 TGACTGATAGGGATAACAAACA TTGTGGGATCGTTTTCTCATGA 36.4 40.9 54.5 58.0 2077-2085 bp 1.0 40.4
ycf3_p5 TGACTGATAGGGATAACAAACA TGGGATCGTTTTCTCATGAGAG 36.4 45.5 54.5 57.9 2074-2082 bp 1.0 40.4