Hotspot

rps19

LSC · 88929-89207 · 279 bp

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Pi 0
MarkerSeek score 19.30
Primer available yes
Label rps19

Feature Metadata

label_name
rps19
feature_type
gene
region
LSC
length_bp
279
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
19.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19_p1 TTGTCCCATAATCCCCCACT AACAGTCGGACAAGTAGGGA 50.0 50.0 58.0 58.0 1176 bp 1.0 80.3
rps19_p2 TTGTCCCATAATCCCCCACT ACAGTCGGACAAGTAGGGAA 50.0 50.0 58.0 58.0 1175 bp 1.0 80.3
rps19_p3 TTGTCCCATAATCCCCCACT GATGAAGGAACGGGAAGACC 50.0 55.0 58.0 58.0 1574 bp 1.0 80.0
rps19_p4 TTGTCCCATAATCCCCCACT CCAGATGAAGGAACGGGAAG 50.0 55.0 58.0 58.0 1577 bp 1.0 80.0
rps19_p5 TTGTCCCATAATCCCCCACT GGGCCCCAATTGGTAGAAAA 50.0 50.0 58.0 58.1 1033 bp 1.0 79.9